A Coulometric Determination of Urea Nitrogen in Blood and Urine.
نویسندگان
چکیده
Since the ammonia undergoes a 3-electron change, 1 mole is equal to 3 equivalents of the generated titrant. Recently we (lescril)ed an improved cud-point (letection for this system and applied it to titration of as little as 1.4 g. ammonia (2). This method has been extended to titration of ammonia formed by the action of urease on urea in blood and urine. In urine samples, ammonia may be titrated directl, before and after action of urease. In the case of blood, the ammonia formed by urease is separated by the well-known microdiffusion technic of Conway (3) which is applicable to series of samples and requires little attention. Frothing, back-suction, bumping, and other difficulties encountered ill distillatioii or aeration procedures of separating ammonia are avoided when microdiffusion is used. While direct nesslerization also bypasses these drawbacks ali(l is rapid, it involves strict adherence to many technical details (4).
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عنوان ژورنال:
- Clinical chemistry
دوره 11 شماره
صفحات -
تاریخ انتشار 1965